Q-Phase is a patented type of holographic microscope with high detection sensitivity
Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.
Telight Q-Phase
Q-Phase is a quantitative label-free live-cell imaging system - ideal if you desire reliable automated segmentation and analysis of cell behavior. It is a holographic microscope optimized for real-time monitoring of living cells with minimal phototoxicity. Q-Phase easily transforms cell features and dynamics into reliable data for your analysis and correlations.
All-steps-in-one
Q-Phase was purposely designed to observe living cells in vitro for long-term several-day experiments. The system creates space for observation, automated, precise, and fast cell segmentation, and thanks to software specifically developed for Q-Phase also analysis – all steps in one system.
Specifications
Microscope
- Microscope configuration: transmission inverted microscope
- Microscopy techniques: holography (quantitative phase imaging), epifluorescence, simulated DIC, brightfield, high-pass filtered phase
- Objectives: magnification 4× to 60×
- Objective turret: 6-position, motorized exchange
- Light source: LED
- Operating wavelength: 660 nm
- Sample stage: motorized, 130 mm × 90 mm travel range
- Focusing: motorized objective turret, 8 mm travel range
- Piezo-focusing: optional, travel range 500 μm
- Lateral resolution: 4 μm with 4× NA 0.1 objective; 0.58 μm with 60× NA 1.4 objective
- Field of view: objective and camera dependent, up to 1.48 mm × 1.48 mm with 4× objective
- Acquisition framerate: 16 fps (higher framerates on request)
- Reconstructed phase image size: 1200 × 1200 px
- Illumination power at sample plane: down to 0.9 mW/cm2
- Phase detection sensitivity: down to 0.011 rad
- Power: 230 V/50 Hz (120 V/60 Hz optional), 1200 VA
- Dimensions (W × L × H): 1100 mm × 950 mm × 1620 mm microscope with incubator, 2515 mm × 974 mm × 1620 mm total with operator table
- Weight: 350 kg (including microscope table, fluorescence module and microscope incubator)
- Field and aperture diaphragms
- Side port available for fluorescence
- Microscope table with anti-vibration suspension
- Control panel with multifunctional touchscreen, sample stage joystick and rotary knobs
- Microscope incubator with computer temperature setting and temperature data logging (optional)
- Incubation chamber for precise and long-term controlof temperature, humidity and CO2 concentrations (optional) module or other additional techniques
Fluorescence Module
- Light engines: Lumencor with 3 channels (optionally up to 5 channels)
- Detectors: Andor Zyla 4.2 PLUS sCMOS (2048 px x 2048 px)
- Filters: 3 multichannel filter cubes, motorized channel switching