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Holomonitor M4
Non-invasive Live Cell Analysis
  • Living in a small corner of your incubator
  • Non-invasive – no labels or toxic stains needed
  • Provides quantitative data on single-cell level
  • Powerful and unique application portfolio
  • Convenient and easy to use
  • Saves time, money, and cells

Accelerate  your cell research

What are your cells doing over time? And over a long time? How do they develop? Where do they go? And on which itineraries? Do they change shape? And to what degree?

Phase Holographic Imaging provides an innovative tool that lets you continuously image and monitor your cells directly inside your incubator even over long periods of time.

The HoloMonitor® M4 is a small microscope designed to operate 24/7, in a small corner of your  incubator. Without any labels or stains, it uses digital holography to record your cells in real-time.

The result is real 3D images and quantitative data on your cultures, all the way down to single-cell level, collected in a completely non-invasive way. And beyond standard data be amazed with all the morphological parameters you can examine this way. Just by looking at cells with a holographic view.  

HoloMonitor M4

HoloMonitor is a time-lapse cytometer for label-free in vitro live cell imaging and analysis, directly in your incubator.

HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living - well, or dying - cells, under physiologically optimal conditions. No stains needed.

A non-invasive incubator microscope

The employed technique, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live-cell kinetic investigations, at both single-cell and cell-population levels.

The HoloMonitor M4 live cell imaging system is designed to visualize and quantify adherent cells over time. The system software, App Suite, allows researchers to create publication-ready images, videos and diagrams of cellular behavior, using a range of applications  - all label-free.


Further Information

One sample - multiple results

The heart of HoloMonitor is the modular App Suite software, with tailor-made apps for different live cell assays. Each app offers a guided setup of your experiment, automatic image collection and real-time data analysis and presentation.

The Applications

• Kinetic Cell Morphology (with 30+ parameters)
• Wound Healing (Scratch assay)
• Cell Growth | Cell Division Cell Cycle
• Cytotoxicity & Cell Death

• In-depth Single Cell Tracking & Analysis
• Chemotaxis
• Cellular Differentiation
• Cell Counter

• Kinetic Cell Motility & Migration
• Kinetic Cell Proliferation
• Kinetic Drug Dose-Response
• Cell Quality Control (QC)


HoloMonitor® M4

  • Light source: External laser unit
  • Sample illumination: 635 nm, 0.2 mW/cm2
  • Objective: 20x
  • Lateral resolution: 1 μm
  • Field of view: 567 μm × 567 μm
  • Working distance: 0.5 – 2 mm
  • Autofocusing range: 1.5 mm
  • Image capture rate: 1 image/s
  • Image size: 1024 × 1024 pixels
  • Stage travel range: 100×70×10 (x×y×z)
  • Stage repeatability: 5 μm
  • HoloMonitor M4® dimensions: 290×200×190 mm (w×d×h)
  • Space required in incubator: 400×270×190 mm (w×d×h)
  • Weight: 5.15 kg


Computer Requirements

  • Operating system: Windows 10, 64–bit
  • Processor: Intel Core i7 (8th Gen)
  • Memory: 16 GB RAM (min 8 GB)
  • Hard drive: 512 GB SSD (min 256 GB)
  • Display: Full HD (min 1920×1080)
  • Other: 2 built–in USB ports


Sample and Environment

  • Cells: Adherent cell monolayer
  • Cell culture vessels: 6-, 24-, 96-well, Petri, IBIDI
  • Operating temperature: 10-40° C
  • Operating humidity: Max 95 %
  • For research use only
The holomonitor live cell assays
Holographic Cytometry Applications Cell QC Assay 3D Live Cell Imaging

Cell QC Assay

Evaluate cell integrity and
detect changes of your cell culture.

Holographic Cytometry Applications Dose Response Assay 3D Live Cell Imaging
Dose Response Assay

Recognize drug toxicity patterns and
kinetics of drug effects from interactive
dose response curves.

Holographic Cytometry Applications Spatial Cell Tracking 3D Live Cell Imaging

Single Cell Tracking

Study single cell motility and migration
patterns and distinguish between cell
subpopulation behavior.

Holographic Cytometry M4 Applications Cell Count 3D Live Cell Imaging

Cell Counter

Determine the exact cell
number in your cell suspension.

Holographic Cytometry Applications Cell Motility Assay 3D Live Cell Imaging

Cell Motility Assay

Explore average cell population motility
based on cell speed and accumulated
cell distance in real–time.

Holographic Cytometry Applications Kinetic Morphology 3D Live Cell Imaging
Kinetic Morphology

Examine more than 30 parameters,
as cell morphology can be sensitive
markers for cell damage.

Holographic Cytometry Applications Cell Proliferation Assay 3D Live Cell Imaging

Cell Proliferation Assay

Robust kinetic cell proliferation
assessment by direct identification and
counting of individual cells.

Holographic Cytometry Applications Woundhealing 3D Live Cell Imaging
Wound Healing Assay

Easily obtain kinetic information on how
cell populations close the wound gap and
determine cell front velocity

Culture Vessel Holders

HoloMonitor’s motorized stage supports a variety of culture vessels, each secured with a vessel holder. This means easy attachment and exact positioning on the microscope.


HoloLids are specially designed lids for standard cell culture vessels. They are optically superior to normal vessel lids and ensure the best possible image quality every time.


HoloDry is a non-toxic canister that is easily attached to the HoloMonitor microscope. It keeps the optics and interior free of condensation, which ensures optimal performance.

Buy Holomonitor M4 Consumables in our Webshop!